We are seeking a talented and highly motivated biologist/pharmacologist to join the Gastrointestinal and Metabolic therapeutic area in the Discovery Research department. This individual will contribute to the advancement of a research and early development pipeline of innovative therapeutics for unmet medical needs. The ideal candidate will be a highly motivated biologist to design and perform laboratory experiments primarily related to cellular functional assays to characterize biologics and small molecules of interest.
- Employee will design and establish experimental approaches addressing mechanism of action of drug candidates such as cellular uptake and kinetics, localization and recycling of drug product within target cells and draw conclusions and develop hypotheses and design experiments to test hypotheses based on data.
- Routine bioanalysis of in vivo samples by immunoassay or enzyme assay will be required at times.
- Employee may be expected to work with laboratory animals and perform in vivo experiments on models of human disease.
- Manage external CROs to supplement in-house in vitro and in vivo efforts
- Evaluate new target opportunities and construct target validation work plans
- Prepare scientific reports, put own data in context with literature, and participate in team-wide scientific presentations
Responsibilities: (% of time):
Design and perform experiments to develop in-vitro and cell based assays, working with different mammalian cell lines, purified proteins, or in vivo samples. Skills with imaging, colorimetric/fluorimetric assays and classical molecular biology techniques required (DNA/RNA and protein analysis tools)
Analyze, interpret and summarize data; prepare data for presentation and reports
Perform routine literature searches to stay current in disease area biology, Maintain awareness of new laboratory technologies,
Become proficient at operating and maintaining analytical tools of interest
BS/MS in biology related field with 5+ years of industrial pharmaceutical experience in mammalian cell culture or PhD with post-doctoral experience, in vitro cell based assays, enzyme assays, cell culture, immunoassays, electrophoresis, and immunoblot techniques required.Key Skills and Abilities:
- Skills: Hands on experience and proficiency with the use of analytical tools and techniques for performing classic molecular biology and biochemical techniques, mammalian cell culture, colorimetric and fluorimetric assays, ELISA, Western blot, enzymatic activity assays and spectrophotometry.
- Proficient in genetic modification techniques (RNAi, CRISPR, and use of lentiviral or AAV vectors
- Demonstrated capability for high quality work and keen attention to details
- Ability to function independently
- Proficiency in technical writing and presentation
- Strong computer skills including extensive experience with Microsoft Word, Excel, and Power Point
- Recommend specialized equipment (capital and other lab equipment) based on set budget and established time lines designated by department head
- Contribute specific experimental designs and test conditions based on knowledge gained through prior hands-on experience and/or review of relevant technical literature
- Recommend specific experimental reagents and vendors based on familiarity, preference, availability and price
Location/Region: Cambridge, MA